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Sensitive methods for the detection and analysis
of rare mRNA transcripts or other RNAs present in low abundance are
an important aspect of most cell/molecular biology studies. RNA
cannot serve as a template for PCR, so it must first be reverse
transcribed into cDNA [e.g. with reverse transcriptase from Moloney
murine leukemia virus (M-MuLV) or avian myeloblastosis virus (AMV)].
Powell et al. first described a combined technique (now commonly
known as RT-PCR, see figure below) in which reverse transcription
(RT) is coupled with PCR amplification of the resulting cDNA.
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